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. 2021 Sep 23;13(10):1547. doi: 10.3390/pharmaceutics13101547

Figure 2.

Figure 2

Cell type dependent uptake of EV-ZnPc. (a) MC38, D1DCs, or RAW cells were treated with ZnPC alone or EV-ZnPc for 24 h prior to fluorescent detection by flow cytometry using Annexin-V/DAPI staining. MC38-CFP cells were co-cultured with either D1DCs or RAW cells and incubated with EV-ZnPc as described in methods. The fluorescence in the subpopulations was detected by flow cytometry. Gating was performed to exclude debris (not shown), (b) single cells and (c) dead cells. (d) CFP and CD11b/c were used to distinguish the uptake between (e) tumor cells and D1DCs or (f) tumor cells and macrophages. Data are means ± SEM (n = 3). **** p < 0.0001; *** p < 0.001; * p < 0.05.