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. 2021 Sep 22;10(10):1976. doi: 10.3390/plants10101976

Table 1.

Primer sequences used for the amplification of housekeeping and candidate genes studied in the multi-factorial system Pinus radiata-Fusarium circinatum-Phytophthora species.

Gene Primer Sequence GenBank ID Functions/Putative Functions References
Chitinase (PR3) F: TGGCAACACGGACGCCCATT HM219849.1 Hydrolyzation of chitin. [45,50,73,74]
R: ACCGGCGTCGTTTCTGTGCTT
Thaumatin-like protein (PR5) F: AGGAGCGCGTGTGATGCGTT JQ015859.1 Involved in cell wall damage and formation of pores on the plasma membrane. [45,46,47,48,49,50,75,76]
R: TGAAAGTGCTGGTGGCGTCGT
Phenylalanine ammonia-lyase (PAL) F: TGCTGGCCACTGTGAAGCAGA AY641535.1 Lignin and phenolic accumulation in plants. Cinnamic acid synthesis [45,46,47,48,49,50,77,78]
R: TCGCAGAAACGGCCTGGCAA
Pyruvate decarboxylase (PDC) F: CCCGCAAACAATGACGTGGGGT JQ264496.1 Involved in aerobic fermentation. [45,46,47,48,49,50,79,80]
R: TGCGAGCAGATGGTCCAGCA
Actin (ACT) F: TGGACCTTGCTGGGCGTGATCT GQ339779.1 Major component of cytoskeleton
microfilaments.
[45,46,47,48,49,50,81]
R: ACAATCTCGCGCTCTGCGGT
β-Tubulin (TUB) F: AAGGGGGTCAGTGTGGCAACCA KM496536.1 Structural units of the cytoskeleton microtubes [45,46,47,48,49,50,82]
R: ACAGCCCGCGGAACAAACCT
Ubiquitin (UBQ) F: AGCCCTTATGCCGGAGGGGTTT AF461687.1 Participates in protein recognition by the proteasome [45,47,50]
R: AGTGCGGGACTCCACTGTTCCT