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Effectiveness of Rut in PI3K/Akt/GSK3β phosphorylation in platelets. Washed platelets were preincubated with 0.1% DMSO or Rut (2.5 and 5 µM) and then treated with collagen (1 μg/mL) for immunoblotting (A) PI3K, (B) Akt, and (C) GSK3β phosphorylation. Data are expressed as the mean ± standard error of the mean (n = 4). ** p < 0.01 and *** p < 0.001 compared with the resting control; # p < 0.05, ## p < 0.01, and ### p < 0.001 compared with the 0.1% DMSO-treated group.
