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(A) False-color intensity-based image of bacterial biofilm. (B top) Overlap of the phasor plots of standard porphyrins PPIX (pink circle: methanol solution, green circle: crystal) and CPI (cyan circle: methanol solution, blue circle: crystal) and biofilm sample (red circle: bacteria, yellow circle: biofilm matrix). (B bottom) Phasor images of the standards, color code as in the plot. (C) Corresponding phasor image of the biofilm in A. Scale bar in A and C: 25 μm.
