Figure 5.

AVL stimulated the Raf /ERK signaling pathway in Hela S3 cells. (a) The expressing levels of c-Raf, p-c-Raf, ERK and p-ERK were detected by Western blot; (b) Quantification of Western blot; (c)the virus titers were determined in Hela S3 cells treated with Sorafenib; (d) the virus titers were determined in Hela S3 cells treated with U0126. Virus titers were determined by TCID50 assay in HEK293A cells. Data were presented as the mean ± SEM from at least three independent experiments. (* p < 0.05).