Figure 2.

Behavioral analyses of 5 dpf s1r mutant larvae. (A) Analysis of the distance traveled by the larvae during the light/dark (intensity 100%/0%) cycle in the VMR test. The activity was measured for 70 min with a training phase of 30 min in the dark (OFF 0%), then two cycles of light/dark (ON 100%/OFF 0%) of 10 min each. The graph shows the distance traveled per min for s1r^WT and s1r^+25/+25 larvae. (B) Relative distance traveled during the training phase [blue dotted lines in (C), between 21 and 29 min]; (A) during the ON phase [the averaged ON1 and ON2 phases]; (D) during the OFF phase [the averaged OFF1 and OFF2 phases]. Relative distance was expressed as % of controls. (E) Illustration of the OKR test. (F) Quantification of the number of saccades performed in 2 min. (G) Illustration of the touch escape test. The larva was placed in a rail filled with E3 medium, the tail was touched with a tip and the distance was measured. (H) The distance traveled was measured during 5 s, repeated three times per larva and averaged. Data show mean ± SEM from three replicas. The number of animals is indicated within the columns. * p < 0.05, ** p < 0.01; unpaired t-test.