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. 2021 Oct 13;22(20):11049. doi: 10.3390/ijms222011049

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Related mRNA and protein expression levels of ER stress markers in zebrafish larvae in physiological conditions at 5 dpf. (A) mRNA levels were analyzed by qPCR in whole larvae. (B) Protein contents were analyzed by Western blot. (C) Representative Western blots. zeif2α level and Stain-Free^™ were used as a loading control in qPCR and Western blot analyses, respectively. Data are expressed as mean ± SEM, normalized to s1r^WT control level with n = 5–9 in each group. * p < 0.05, ** p < 0.01, *** p < 0.001; unpaired t-test.