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. 2021 Oct 13;22(20):11049. doi: 10.3390/ijms222011049

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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(A) Effect of the Tunicamycin (2 µg/mL, 24 h) challenge on ER stress gene expression levels in s1r^+25/+25 zebrafish larvae at 5 dpf. The selected genes were analyzed using cDNA prepared from whole zebrafish larvae and relative mRNA expression were expressed as percentage of s1r^WT + 0.1% DMSO controls. The fold change from 0.1% DMSO treatment is indicated. Data are expressed in mean ± SEM of n = 5 determination in each group. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. DMSO treatment; ° p < 0.05, °° p < 0.01, °°° p < 0.001 vs. s1r^WT line; two-way ANOVA followed by unpaired t-test. (B) Schematic summary of ER stress pathway alterations observed in basal condition or after tunicamycin treatment in s1r^+25/+25 zebrafish line.