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. 2021 Oct 12;13(10):1668. doi: 10.3390/pharmaceutics13101668

Figure 2.

Figure 2

Evaluation of the ability of mild MHT to induce cell death in U87 cells measured using Annexin V/PI assay. MHT induces reduction of cell viability in U87 (a). After being exposed to mild MHT (3 cycles of 30 min at 43 °C), U87 cells were left to rest for 24 h and then stained with annexin-V-FITC and PI. Frequency of apoptotic (Annexin V+/PI−) (blue bars), primary necrotic (Annexin V-/PI+) (grey bars) and secondary necrotic (Annexin V+/PI+) (red bars) U87 cells were determined via flow cytometry (b,c). Data are represented as mean ± SD of three independent experiments (n = 3) (b). Representative dot plots of Annexin V/PI staining of U87 cells treated with AMF only (AMF), IONCs only (IONCs), heat bath at 43 °C (HT) or IONCs + AMF (MHT) (c). Statistical significance was determined with a two−tail unpaired student’s t test (** 0.001 < p < 0.01; *** p < 0.001; n.s. = not significant).