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. 2021 Oct 12;13(10):1668. doi: 10.3390/pharmaceutics13101668

Figure 3.

Figure 3

Evaluation of the clonogenic (a) and migration (b) activity, surface expression of EMT (c,d) and CSC markers (e) in U87 cells in response to treatment with IONCs and mild MHT (3 cycles of 30 min at 43 °C). Clonogenicity was evaluated in U87 cells after treatment with AMF, IONCs and MHT (a). Data are shown as representative images of stained colony with crystal violet and relative absorbance at 595 nm. A transwell assay was used to determine the migration and invasion ability of U87 cells after exposition to different treatments such as AMF only (AMF), IONCs only (IONCs) and IONCs + AMF (MHT) (b). The results are represented as relative percentage compared to untreated cells (d). Error bars indicate ± SD calculated from three independent experiments (n = 3). Surface expression of E-cadherin, Vimentin and CD133 was measured via flow cytometry at 24 h post-treatment with AMF, IONCs and MHT, and data reported relative to untreated controls (c–e). Data are represented as mean ± SD of three independent experiments (n = 3). Statistical analysis was conducted with a two−tail unpaired student’s t test (*** p < 0.001; n.s. = not significant).