Table 5.
The composition of the control and FLI medium.
| Component | Supplier | Control Medium | FLI Medium |
|---|---|---|---|
| TCM199 | Sigma, M7528 | TCM199 | TCM199 |
| Sodium pyruvate | Sigma, P4562 | 0.2 mM | 0.2 mM |
| L-glutamin | Sigma, G8540 | 6.85 mM | 6.85 mM |
| Cysteine | Sigma, C7352 | 0.57 mM | 0.57 mM |
| Gentamycin | Roth, 0233 | 50 µg/mL | 50 µg/mL |
| BSA | Sigma, A7030 | 1 mg/mL | 1 mg/mL |
| PMSG | Prospec 1, HOR-272 | 10 IU/mL | 10 IU/mL |
| hCG | Prospec 1, HOR-250 | 10 IU/mL | 10 IU/mL |
| EGF | PeproTech 2, AF-100-15 | 10 ng/mL | 10 IU/mL |
| human LIF | Merck 3, LIF1005 | − | 2 µL/mlL |
| human IGF1 | PeproTech 2, AF-100-11 | − | 20 ng/mL |
| human FGF2 | Sigma, F0291 | − | 40 ng/mL |
1 Prospec, Rehovot, Israel; 2 PeproTech, London, England; 3 Merck Life Science, Prague, Czech Republic.