Figure 3.
The effects of CaSR agonist (AC) and CaSR antagonist (NPS) on bladder strips contraction. The recording of contraction in response to the first and the second time of acetylcholine stimulations (sti) (from 1 × 10−7 to 1 × 10−3 M) in each group, which were added with different treatments before the second time of stimulations. (a) Contraction responses before and after added with buffer (control) (strip 1, intact strips) or 0.1 mM AC (strip 2, intact strips) or 0.1 mM AC (strip 3, urothelium removal strips). (d) Contraction responses before and after added with buffer (control) (strip 1, intact strips) or 0.1 mM NPS (strip 2, intact strips) or 0.1 mM NPS (strip 3, urothelium removal strips). The statistical analysis of contraction curves affected by AC (b,c) and NPS (e,f) in intact and urothelium removal strips (Two-way ANOVA with Sidak’s post hoc tests, n = 6 for each group; * p < 0.05, ** p < 0.01, *** p < 0.001, as compared with the control group).