. 2021 Oct 19;22(20):11254. doi: 10.3390/ijms222011254

Table 1.

Mouse primers and PCR conditions used in this study.

Gene *	Primer Sequence (5′-3′)	Size	T_m
IGF-1	F: 5′-GGACCAGAGACCCTTTGCGGG-3′	171 bp	63 °C
IGF-1	R: 5′-GGCTGCTTTTGACCCTTCAGTGG-3′	171 bp	63 °C
IGF-1R	F: 5′-ACTGACCTCATGCGCATGTGCTGG-3′	198 bp	63 °C
IGF-1R	R: 5′-CTCGTTCTTGCGGCCCCCGTTCAT-3′	198 bp	63 °C
GAPDH	F: 5′-ACCACAGTCCATGCCATCAC-3′	480 bp	63 °C
GAPDH	R: 5′-CACCACCCTGTTGCTGTAGC-3′	480 bp	63 °C

* IGF-1, Insulin-like growth factor-1; IGF-1R, Insulin-like growth factor-1 receptor; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; F, forward; R, reverse; bp, base pair; T_m, melting temperature.