Table 1.
Time- and dose-dependent effects of CAC-717 on SARS-CoV-2 viability.
| Reagent | Ratio 1 | Virus Titers (TCID50/mL) | |||
|---|---|---|---|---|---|
| 15 s | 30 s | 60 s | 300 s | ||
| CAC-717 | 1:9 | ≤1.0 ± 0.4 2 | ≤0.8 ± 0.2 * | ≤0.7 ± 0.2 | ≤0.6 ± 0.1 |
| 1:49 | ≤0.6 ± 0.1 | ≤0.7 ± 0.1 | ≤0.6 ± 0.1 | ≤0.5 ± 0.1 | |
| 1:99 | ≤0.7± 0.1 | ≤0.7 ± 0.1 | ≤0.6 ± 0 | ≤0.6 ± 0 | |
| Control | 1:49 | ND | ND | ND | 5.0 ± 0.1 |
| HEPES-treated CAC-717 3 | 1:49 | ND | ND | ND | 4.8 ± 0.2 |
| Alcohol disinfectant | 1:9 | ≤0.5 ± 0 | ≤0.5 ± 0.1 | ≤0.5 ± 0 | ≤0.5 ± 0 |
The titer of virus (SARS-CoV-2/KH-1/2021) used in this experiment was 106.8 ± 0.3 TCID50/mL. 1 Twelve microliters of virus solution was mixed with 9, 49 or 99 volumes of reagent. For control, 49 volumes of distilled water was used. 2 Data are mean ± standard deviation of four independent experiments. The maximum possible viral titers are shown. 3 HEPES neutralized the alkalinity of CAC-717, and the pH was changed from 12.4 to 7.6. * A significant difference as compared with alcohol disinfectant (p < 0.05).