Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Oct 12;8(10):229. doi: 10.3390/vetsci8100229

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Immunohistochemistry of c-kit, Ki67, and CB2 receptor with toluidine blue as the counterstain of atopic dermatitis (AD), cutaneous mastocytosis, and cutaneous mast cell tumors (MCTs). Immunohistochemistry of c-kit of AD (A), mastocytosis (B), and MCTs (C) showed cytoplasmic (black arrow) and membranous (red arrow) (A,B) and only membranous (black arrow) (C) c-kit expression of mast cells (MCs). Immunohistochemistry of Ki67 of mastocytosis (E) and MCTs (F) showed nuclear expression of MCs (E,F) visualized by toluidine blue (TB) stain, and MCs in AD were not in cycle; therefore, Ki67 stain was found only on the epithelial cells of hair follicles (black arrows) (D). Mast cells of mastocytosis (H) and MCTs (I) revealed nuclear expression of CB2 receptor (black arrows); by contrast, the MCs of AD (G) showed cytoplasmic expression of the CB2 receptor (black arrow) that hid the metachromatic reaction of TB stain. Scale bar = 50 µm.