Figure 2.

The multi-drug resistant cells exhibit features of the tumor-initiating cells. (A) Cells were stained with ALDEFLUOR for assessment of ALDH expression. The percentage of the gated area was annotated. (200r was used for the study of resistant FaDu; * p < 0.05.) (B) Western blot analysis of the expression of different stemness biomarkers in the parental and the drug-resistant cells was studied for the protein expression. (200r was used for the study of resistant FaDu). (C) Expression of the RNA transcripts of various stemness-related genes was examined by qPCR in the parental and the two FaDu resistant cells (200r and 1000r).