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. 2021 Oct 12;22(20):11012. doi: 10.3390/ijms222011012

Table 1.

Primer sequences for the genes of interest and reference gene.

Primers Direction Sequences
TNF-α Forward 5′-AGCAAACCACCAAGTGGAGGA-3′
Reverse 5′-GCTGGCACCACTAGTTGGTTGT-3′
CCL-2 Forward 5′-GAAGGAATGGGTCCAGACAT-3′
Reverse 5′-ACGGGTCAACTTCACATTCA-3′
IL-6 Forward 5′-GCTACCAAACTGGATATAATCAGGA-3′
Reverse 5′-CCAGGTAGCTATGGTACTCCAGAA-3′
IL-10 Forward 5′-ACTGGCATGAGGATCAGCAG-3′
Reverse 5′-CTCCTTGATTTCTGGGCCAT-3′
18s Forward 5′-GCAATTATTCCCCATGAACG-3′
Reverse 5′-GGCACTTAATCAACGCAAGC-3′

TNF-α, tumor necrosis factor-alpha; CCL-2, C-C motif chemokine ligand-2; IL-6, interleukin-6; IL-10, interleukin-10; 18 s, 18 s rRNA.