Figure 3.

BL protects against AOM/DSS-induced intestinal barrier dysfunction. (A) Intestinal permeability, and (B,C) the expression of zonula occludens-1 (ZO-1) and occludin in the colonic tissues (n = 8–10). (D) AB staining and colonic goblet cell numbers were quantified (400×). (E) Immunofluorescent analysis of Muc-2 (red) in mouse colonic sections (400×). Data are mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001. The statistics were performed with one-way ANOVA. BL, barley leaf; AOM, azoxymethane; DSS, dextran sodium sulfate.