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. 2021 Oct 17;14(10):1055. doi: 10.3390/ph14101055

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Effect of T. officinale and C. intybus extracts on SARS-CoV-2-Spike–ACE 2 inhibition. (A,B) Concentration-dependent effect of T. officinale (TO) and C. intybus (CI) extract. (C,D). Effect of fractions from TO and CI leaf extract. The extracts were freeze-dried and a molecular weight fractionation was subsequently carried out. The cut-off was set to 5 kDa (HMW > 5 kDa, LMW < 5kDa). H+L: HMW and LMW fractions; 50 mg of dried leaves per mL water was used as reference. HMW and LMW fraction quantities equivalent to dried leaves were used. The binding inhibition was assessed using ELISA technique. Bars are means + SD. Solvent control: distilled water (a.d.); * p < 0.05, ** p < 0.01. Significance of difference was calculated relative to the solvent control by one-way ANOVA.