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. 2021 Oct 14;11(10):1084. doi: 10.3390/life11101084

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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CD133 and CD44 expression profiles and their co-expression in MG1, BR1.20, BR3.20, U343 MG, and U87 MG cell cultures. Cell suspensions were labeled with PE-conjugated anti-CD133 and APC-conjugated anti-CD44 antibodies and analyzed by flow cytometry. The top portion (Q1, Q2) is CD44-positive; the right portion (Q2, Q3) is CD133-positive cells. The double-positive cells in the right-top quarter (Q2) are numbered as a percentage of the total cells. The purple arrow defines a decrease in the sensitivity of the cells of the studied cultures to VV-GMCSF-Lact.