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. 2021 Oct 15;22(20):11133. doi: 10.3390/ijms222011133

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Interaction between CsCDPK6 and CsSAMS1. (A) Y2H assays showing the interaction between CsCDPK6 and CsSAMS1. (B) BiFC assay confirming the interaction between CsCDPK6 and CsSAMS1 in vivo. YFP field indicates fluorescence signals. Scale bar = 100 μm. (C) Co-immunoprecipitation assay. Immunoblot analysis confirmed the expression of input proteins: CsSAMS1-HA (panel 1, anti-HA antibody), CsCDPK6-Flag (panel 2, anti-Flag antibody); the IP assay was incubated with anti-HA-tag magnetic beads, and CsCDPK6-Flag was detected only after co-immunoprecipitation with the sample expressing CsSAMS1-HA (panel 3, anti-FLAG antibody).