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HEK NA deglycosylation analysed by Western blot. (a) The supernatant of a pTT5-NA (+SS −TET) and lysed pellet of pTT5-NA (−SS −TET) were digested with deglycosylation enzymes The digested proteins were separated by SDS-PAGE, transferred to PVDF membrane for analysis by Western blot. An anti-His antibody was utilised to visualize the NA proteins. (b) Proteins were denatured prior to deglycosylation, and then deglycosylated and analysed on WB in the same way as in panel a.
