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Purification of NA following HEK cell expression. SDS-PAGE (4–20%) separation of proteins obtained following key steps in NA purification. Lane 1: Mol Wt. standards. Lane 2: Whole cell lysate. Lane 3: 10× concentrated supernatant following expression. Lanes 4–6: His trap, HIC and SEC samples. Proteins were visualized using Coomassie Blue.
