Table 2.
NA Purification.
| Step | Volume (mL) | Purity (BIORAD) | [Protein] (μg/mL) | Total Protein (μg) | Total NA (μg) | Yield (%) a |
NA Activity (EU/mL) | Total EU | Specific Activity (EU/μg) b | Fold Purification c |
|---|---|---|---|---|---|---|---|---|---|---|
| Supernatant | 1000 | 14 | 199 | 198,700 | 27,818 | 100 | 3.7 × 103 | 3.7 × 106 | 18 | 1 |
| His-Trap | 66 | 47 | 260 | 17,173 | 8071 | 29 | 1.9 × 104 | 1.2 × 106 | 75 | 4 |
| HIC | 9 | 100 | 80 | 727 | 727 | 2.6 | 2.7 × 104 | 2.4 × 105 | 336 | 18 |
| SEC | 1.5 | 100 | 269 | 403 | 403 | 1.4 | 1.9 × 105 | 2.8 × 105 | 710 | 38 |
a Yield was calculated using the final total NA activity divided by the initial total NA found in the supernatant prior to purification. b The specific activity was calculated by dividing total EU by total protein. c Fold purification was calculated by dividing the specific activity by the specific activity of the supernatant.