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. 2021 Sep 29;9(10):1111. doi: 10.3390/vaccines9101111
Essential components of ADCS
  • 1 

    ANTIBODIES

Types Considerations for Abs Considerations for target antigen

  1. Monoclonal Ab (chimeric, humanized or human, mainly IgG1 isotype)

  2. Bispecific Ab

  • Specificity and affinity for the target antigen

  • Internalization

  • Pharmacokinetic properties

  • Effector functions

  • Immunogenicity

  • Tumour-specificity

  • Internalization

  • Expression pattern

  • Ectodomain shedding
  • 2 

    LINKERS

Types Considerations for linker Considerations for conjugation

  1. Cleavable linkers

  2. Non-cleavable linkers

  • Circulation stability

  • Solubility

  • Aggregation propensity

  • Drug to antibody ratio (DAR)

  • Homogeneity
  • 3 

    PAYLOADS

Types Considerations for payload Mechanisms of drug resistance

  1. Calicheamicins

  2. Pyrrolobenzodiazepines (PBDs)

  3. Auristatins

  4. Maytansinoids

  5. Camptothecin (CPT)

  • Half life

  • Bystander killing activity

  • Systemic accumulation

  • Potential for resistance development

  • Antigen-related resistance

  • Deficient lysosomal function

  • Upregulated efflux pumps

  • Survival/apoptotic signaling

  • BSB phenomenon