Figure 4.

Deubiquitinase activity of USP38 is required for restriction of ZIKV infection. (A) HEK293T cells were co-transfected with Flag-E and Myc-UB, together with HA-USP38 or HA-USP38 (C454A/H857A/D918N). Cell lysates were immunoprecipitated with anti-Flag and immunoblotted with anti-Myc. (B–E) Hela cells were transfected with HA-USP38, HA-Vector or HA-USP38 (C454A/H857A/D918N) for 24 h then infected with ZIKV (MOI = 1) for 48 h. The expression level of ZIKV proteins were detected by immunoblotting (B). The ratios of E/GAPDH and NS5/GAPDH ratio were measured (C). The viral RNA content was quantified by qPCR (D) and confocal microscopy (E), bar = 10 µm.