Figure 6.

Effect of GCSF on the expression levels of testicular growth factors and pro-inflammatory cytokines of AML- and CYT-treated mice. Mice were treated as described in Figure 2. The RNA expression levels of the growth factors GDNF (A), SCF (B), MCSF (C) and the pro-inflammatory cytokines IL-1 alpha and IL-1 beta (D) in testes of all examined groups were tested by qPCR analysis using specific primers for each factor. qPCR results are presented as fold increase compared to the control group (CT). The results are representative of 3 independent experiments with 3 mice in each group per experiment. *—significant compared to control (CT). #—significant compared to AML, &—significant compared to CYT, $—significant compared to AML + CYT. %—significance of AML + CYT compared to CYT. *,#,&,$,%—p < 0.05; **,##,&&,$$—p < 0.01; ***,###,$$$—p < 0.001.