Skip to main content
. 2021 Oct 3;9(10):2090. doi: 10.3390/microorganisms9102090

Figure 1.

Figure 1

AnxA2 mediates the invasion of S. aureus. (A) Transmission electron micrograph showing internalization of a clinical isolate of S. aureus (Clin-SA) into MAC-T. Two engulfed bacteria (boxed) are displayed at high magnification. Photograph represents a 1 h co-culture of cell monolayer with Clin-SA at a MOI of 50. N: nucleus; C: cytoplasm. Scale bar: 500 nm. (B–D) Effect of AnxA2 on S. aureus adhesion to and uptake by MAC-T cells. MAC-T cells were pre-incubated with anti-AnxA2 antibody (17.5 μg/mL) or human IgG (17.5 μg/mL) for 2 h to block AnxA2 function (B), treated with AnxA2 siRNA for 72 h to knock down the expression of AnxA2 (C), or transfected with a pEGFP-AnxA2 plasmid for 48 h to overexpress AnxA2 (D) prior to Clin-SA infection (MOI: 50). The adhesion and invasion assays were performed after incubation of MAC-T with bacteria for 1 h. Dates are mean values ± s.d. of three replicates. * p < 0.05; ** p < 0.01; ns: no significant difference.