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. 2021 Oct 3;9(10):2090. doi: 10.3390/microorganisms9102090

Figure 2.

Figure 2

Engagement of AnxA2 in S. aureus-induced cytoskeleton reorganization. MAC-T cells were pre-treated with cytochalasin D (Cyt D) for 2 h (A–C) or AnxA2 siRNAfor 72 h (D), followed by infection with clinical isolate of S. aureus (Clin-SA, FITC-labeled) or S. aureus strain ATCC 25923 for 1 h. F-actin was visualized by TRITC-conjugated phalloidin (A,D). Representative confocal microscopy images of cells infected with Clin-SA are shown (Green: S. aureus; Red: F-actin). Invasion of Clin-SA (B) as well as ATCC 25923 (C) was determined by gentamicin protection assay. The graph shows mean values ± s.d. of three replicates. Each assay was repeated at least three times. ** p < 0.01 and *** p < 0.001.