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. 2021 Oct 3;9(10):2090. doi: 10.3390/microorganisms9102090

Figure 5.

Figure 5

Binding of S. aureus ClfB to AnxA2 of MAC-T. (A) GST and GST-tagged recombinant ClfB produced from E. coli BL21 were purified and assessed by SDS-PAGE, and the proteins were visualized by Coomassie brilliant blue staining. (B) GST-ClfB protein and GST protein were combined to Sepharose CL-6B beads, and incubated with whole cell lysates prepared from MAC-T. The protein complexes were released by boiling, electrophoresed on 12% SDS-PAGE, and subjected to Western blot analysis with anti-AnxA2 antibody.