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. Author manuscript; available in PMC: 2022 Feb 28.
Published in final edited form as: Sci Signal. 2021 Aug 31;14(698):eabc1044. doi: 10.1126/scisignal.abc1044

Fig. 2. GPCR agonists stimulate p38-dependent HSP27 phosphorylation in different endothelial cell types.

Fig. 2.

(A and B) Left: Immunoblotting analysis of phosphorylated HSP27 and p38 induced by 10 nM thrombin (α-Th) at the indicated times in (A) HUVECs or (B) HDMECs pretreated for 1 hour with 3 μM SB203580 or DMSO. (C) Left: Immunoblotting analysis of phosphorylated HSP27 and p38 induced by 10 μM histamine at the indicated times in HUVECs pretreated with SB203580 or DMSO. Blots in (A) to (C) are representative of four independent experiments. (A to C) Right: Densitometric analysis of the fold-change in the relative abundances of the indicated phosphorylated proteins over time. Data are means ± SD of four independent experiments and were analyzed by Student’s t test. *P < 0.05; **P < 0.01; ***P < 0.001.