Figure 1.
Summary of the present study. Kit-Met 1 and Kit-Met 2 were performed by the Xevo® TQ-S and Xevo® TQ-XS MS system (Waters, Wilmslow, Manchester, UK), respectively. Both MS systems were conducted with the UHPLC system, which consisted of dual pumps (ACQUITY UPLC H-Class, Waters) and an autosampler with a column compartment (ACQUITY UPLC I-Class, Waters). NMR, nuclear magnetic resonance; UHPLC-MS/MS, ultrahigh-performance liquid chromatography triple quadrupole tandem mass spectrometry; FIA-MS/MS, flow injection analysis MS/MS; SFC-MS/MS, supercritical fluid chromatography MS/MS; UHPLC-FTMS, UHPLC Fourier transform MS. *1 Metabolites in human plasma NIST® SRM® 1950 (Merck- Sigma-Aldrich, Parts No.: NIST1950). *2 Pooled normal human plasma Na EDTA (Innovative Research, Parts No. IPLA-N, Lot 26393).