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. 2021 Oct 6;10(10):1286. doi: 10.3390/pathogens10101286

Figure 2.

Figure 2

Killing activity of curry leaf extract. (Left panel) P. gingivalis cells were treated with curry leaf extract (CLE) at MIC (16 μg/mL) or the vehicle control (ethanol) for 30 or 120 min. Ampicillin and tetracycline were also used at concentrations of 100 μg/mL (ca. 6000-fold MIC) and 5 μg/mL (not less than 100-fold MIC), respectively. Viability of P. gingivalis was evaluated by counting CFUs on BAPs after culture for 14 days. The survival rate is shown as (CFU [tested sample]/CFU [vehicle control]) × 100 (%) at each time point. Data are expressed as the mean with SD from results obtained in three independent experiments. NS: no statistical significance. ** p ≤ 0.01, **** p ≤ 0.0001 (vs. vehicle control). (Right panel) P. gingivalis cells were treated with CLE at the concentrations ranging from 1 to 32 µg/mL, or the vehicle control (1% ethanol) for 30 and 120 min. Shown are the survival rates after treatment, compared to the vehicle control. The rate denoted as CFU (%) was calculated as (CFU [tested sample]/CFU [vehicle control]) × 100 (%).