Table 1.
Primers used for qRT-PCR in S. frugiperda.
| Gene | GenBank Accession Number | Primer Sequences (5′-3′) | Length (bp) | Amplification Efficiency (%) | Regression Coefficients |
|---|---|---|---|---|---|
| 18S | KY554596 | F: GACTCAACACGGGAAATCTC R: CCACGCACACCTAAATGAC |
185 | 102.6 | 0.995 |
| AK | KC262642 | F: TCTACCACAACGAGAACAAGAC R: AAAGTGAGGAAACCAAGCC |
180 | 97.88 | 0.992 |
| RPL10 | XM_035580073 | F: TGGGTAAGAAGAAGGCTACG R: TGTTGATGCGGATGACAT |
194 | 104.2 | 0.994 |
| RPS24 | XM_035577015 | F: CACTGGCTTTGCTCTCATC R: TCATCCTGTTCTTGCGTTC |
136 | 102.5 | 0.995 |
| 28S | XM_035580587 | F: GACCAGATTCCGATTTCACT R: CTTTGTTTACCGCTTGCC |
122 | 94.1 | 0.996 |
| SOD | XM_035587671 | F: TCGGCACAATCATCAGTC R: AGTCCTTCTCAATAGCCTGC |
137 | 93.6 | 0.995 |
| ATP | XM_035575197 | F: AAAGTAGTTCCGTGGAGTGAG R: AAAGAAGGGTCCGTAGATTG |
115 | 107.1 | 0.994 |
| GAPDH | XM_035587881 | F: AGAAGACTGTTGACGGACC R: AGGAATGACTTTGCCGAC |
112 | 103.9 | 0.984 |
| ACT | KT218672 | F: TTCTTCCACCCTGAGTTCTC R: AGTCCTCTTGATGTCACGC |
182 | 90.6 | 0.999 |
| a-TUB | XM_035600854 | F: AGGGCTGTGTTTGTTGACT R: TCCTTACCGATGGTGTAGTG |
148 | 93.6 | 0.999 |
| OBP1 | XM_035578840 | F: GTGGGTGTGAGAGAGATAGAAC R: GAACAGGTCTGCTATGATGTG |
125 | 100.0 | 0.994 |