Table 2.
Expression stability of 10 candidate reference genes of S. frugiperda in different developmental stages, tissues, and mating status.
| Treatments | Genes | Delta Ct | geNorm | NormFinder | BestKeeper | ||||
|---|---|---|---|---|---|---|---|---|---|
| Stability | Ranking | Stability | Ranking | Stability | Ranking | Stability | Ranking | ||
| Developmental stages | 18S | 1.282 | 9 | 0.954 | 6 | 0.680 | 7 | 0.352 | 1 |
| AK | 1.236 | 7 | 1.081 | 8 | 0.683 | 8 | 1.053 | 7 | |
| RPL10 | 0.885 | 2 | 0.607 | 3 | 0.174 | 1 | 0.822 | 3 | |
| RPS24 | 1.061 | 3 | 0.444 | 1 | 0.453 | 3 | 0.825 | 4 | |
| 28S | 1.117 | 5 | 0.873 | 5 | 0.625 | 5 | 1.010 | 6 | |
| SOD | 0.813 | 1 | 0.444 | 1 | 0.189 | 2 | 0.904 | 5 | |
| ATP | 1.255 | 8 | 1.143 | 9 | 0.803 | 9 | 1.498 | 10 | |
| GAPDH | 1.207 | 6 | 1.037 | 7 | 0.677 | 6 | 1.104 | 9 | |
| ACT | 1.065 | 4 | 0.797 | 4 | 0.557 | 4 | 0.807 | 2 | |
| a-TUB | 1.283 | 10 | 1.197 | 10 | 0.820 | 10 | 1.079 | 8 | |
| Tissues | 18S | 1.649 | 9 | 1.168 | 8 | 0.961 | 9 | 1.043 | 2 |
| AK | 1.563 | 8 | 1.251 | 9 | 0.937 | 8 | 1.694 | 9 | |
| RPL10 | 1.058 | 1 | 0.995 | 5 | 0.318 | 1 | 1.084 | 4 | |
| RPS24 | 1.209 | 6 | 1.073 | 7 | 0.580 | 5 | 1.246 | 7 | |
| 28S | 1.964 | 10 | 1.405 | 10 | 1.284 | 10 | 1.752 | 10 | |
| SOD | 1.142 | 3 | 0.802 | 3 | 0.502 | 4 | 1.374 | 8 | |
| ATP | 1.153 | 5 | 0.914 | 4 | 0.587 | 6 | 0.919 | 1 | |
| GAPDH | 1.228 | 7 | 0.625 | 1 | 0.593 | 7 | 1.176 | 6 | |
| ACT | 1.146 | 4 | 1.036 | 6 | 0.499 | 3 | 1.135 | 5 | |
| a-TUB | 1.092 | 2 | 0.625 | 1 | 0.483 | 2 | 1.080 | 3 | |
| Mating status | 18S | 1.182 | 4 | 0.408 | 3 | 0.388 | 3 | 0.268 | 2 |
| AK | 1.062 | 1 | 0.190 | 1 | 0.066 | 1 | 0.338 | 3 | |
| RPL10 | 1.067 | 2 | 0.190 | 1 | 0.181 | 2 | 0.212 | 1 | |
| RPS24 | 1.703 | 8 | 1.177 | 8 | 1.069 | 8 | 1.568 | 10 | |
| 28S | 1.968 | 10 | 1.516 | 10 | 1.496 | 10 | 1.459 | 9 | |
| SOD | 1.182 | 4 | 0.770 | 5 | 0.416 | 4 | 0.913 | 6 | |
| ATP | 1.098 | 3 | 0.556 | 4 | 0.644 | 6 | 0.485 | 4 | |
| GAPDH | 1.765 | 9 | 1.312 | 9 | 1.204 | 9 | 1.220 | 8 | |
| ACT | 1.185 | 6 | 0.896 | 6 | 0.549 | 5 | 1.028 | 7 | |
| a-TUB | 1.401 | 7 | 0.999 | 7 | 0.846 | 7 | 0.633 | 5 | |