Table 3.
Expression stability of 10 candidate reference genes of S. frugiperda under hormone treatment, diet treatment, and temperature treatment.
| Treatments | Genes | Delta Ct | geNorm | NormFinder | BestKeeper | ||||
|---|---|---|---|---|---|---|---|---|---|
| Stability | Ranking | Stability | Ranking | Stability | Ranking | Stability | Ranking | ||
| Hormone treatment | 18S | 0.545 | 7 | 0.210 | 1 | 0.216 | 5 | 0.316 | 3 |
| AK | 0.484 | 2 | 0.210 | 1 | 0.119 | 2 | 0.315 | 2 | |
| RPL10 | 0.502 | 4 | 0.341 | 5 | 0.119 | 2 | 0.338 | 4 | |
| RPS24 | 0.506 | 5 | 0.316 | 4 | 0.162 | 4 | 0.436 | 7 | |
| 28S | 0.675 | 8 | 0.486 | 8 | 0.499 | 8 | 0.554 | 9 | |
| SOD | 0.509 | 6 | 0.409 | 7 | 0.271 | 6 | 0.413 | 5 | |
| ATP | 0.432 | 1 | 0.222 | 3 | 0.106 | 1 | 0.267 | 1 | |
| GAPDH | 0.799 | 10 | 0.631 | 10 | 0.557 | 9 | 0.541 | 8 | |
| ACT | 0.499 | 3 | 0.582 | 6 | 0.271 | 6 | 0.419 | 6 | |
| a-TUB | 0.703 | 9 | 0.563 | 9 | 0.565 | 10 | 0.629 | 10 | |
| Diet treatment | 18S | 1.041 | 4 | 0.808 | 3 | 0.224 | 1 | 0.385 | 1 |
| AK | 1.230 | 6 | 0.694 | 5 | 0.654 | 6 | 0.787 | 6 | |
| RPL10 | 1.034 | 3 | 0.636 | 1 | 0.363 | 2 | 0.562 | 2 | |
| RPS24 | 1.254 | 8 | 0.957 | 5 | 0.652 | 5 | 0.812 | 7 | |
| 28S | 1.713 | 10 | 1.319 | 10 | 1.221 | 10 | 1.239 | 10 | |
| SOD | 0.958 | 1 | 0.636 | 1 | 0.441 | 4 | 0.751 | 4 | |
| ATP | 1.249 | 7 | 1.165 | 9 | 0.745 | 8 | 1.149 | 9 | |
| GAPDH | 1.256 | 9 | 1.024 | 7 | 0.715 | 7 | 1.055 | 8 | |
| ACT | 1.003 | 2 | 0.870 | 4 | 0.416 | 3 | 0.596 | 3 | |
| a-TUB | 1.214 | 5 | 1.100 | 8 | 0.793 | 9 | 0.763 | 5 | |
| Temperature treatment | 18S | 1.127 | 3 | 0.950 | 3 | 0.393 | 2 | 0.810 | 3 |
| AK | 1.294 | 9 | 1.143 | 7 | 0.666 | 5 | 0.820 | 4 | |
| RPL10 | 1.070 | 1 | 1.056 | 5 | 0.331 | 1 | 0.687 | 1 | |
| RPS24 | 1.210 | 5 | 0.871 | 1 | 0.599 | 4 | 1.307 | 8 | |
| 28S | 1.722 | 10 | 1.345 | 10 | 1.109 | 10 | 1.554 | 10 | |
| SOD | 1.190 | 4 | 0.871 | 1 | 0.743 | 9 | 1.270 | 7 | |
| ATP | 1.261 | 8 | 1.229 | 9 | 0.720 | 8 | 1.422 | 9 | |
| GAPDH | 1.244 | 7 | 1.004 | 4 | 0.699 | 6 | 1.202 | 6 | |
| ACT | 1.096 | 2 | 1.100 | 6 | 0.527 | 3 | 0.833 | 5 | |
| a-TUB | 1.224 | 6 | 1.180 | 8 | 0.709 | 7 | 0.752 | 2 | |
| Total | 18S | 1.269 | 5 | 1.025 | 3 | 0.524 | 3 | 0.673 | 1 |
| AK | 1.369 | 8 | 1.173 | 6 | 0.745 | 7 | 0.993 | 3 | |
| RPL10 | 1.096 | 1 | 0.977 | 1 | 0.359 | 1 | 0.715 | 2 | |
| RPS24 | 1.369 | 8 | 1.291 | 9 | 0.752 | 8 | 1.491 | 9 | |
| 28S | 1.750 | 10 | 1.414 | 10 | 1.168 | 10 | 1.722 | 10 | |
| SOD | 1.099 | 2 | 0.977 | 1 | 0.512 | 2 | 1.147 | 6 | |
| ATP | 1.349 | 7 | 1.250 | 8 | 0.732 | 6 | 1.168 | 7 | |
| GAPDH | 1.317 | 6 | 1.217 | 7 | 0.720 | 5 | 1.325 | 8 | |
| ACT | 1.128 | 3 | 1.064 | 4 | 0.532 | 4 | 1.065 | 4 | |
| a-TUB | 1.262 | 4 | 1.136 | 5 | 0.757 | 9 | 1.097 | 5 | |