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. 2021 Oct 12;22(20):11013. doi: 10.3390/ijms222011013

Scheme 1.

Scheme 1

Preparation of sulfonated/carboxymethylated derivatives of BOT. Reaction step (a) ClCH2COOH, isopropanol, aqueous NaOH (20%), room temperature to 60 °C, 4 h, and (b) ClSO3H, pyridine, formamide, −4 °C or 60 °C, 3–26 h. For simplification purposes, the polysaccharide structure was represented without branching.