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. 2021 Oct 9;13(10):1649. doi: 10.3390/pharmaceutics13101649

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Fluorinated PEI-based artificial virus for Cas9 plasmids delivery. (A) Schematic illustration of the multifunctional artificial virus. (B) Transfection efficiency of different nanoparticles loading EGFP plasmids at 24 h. The images were taken by fluorescence microscopy in SKOV3 cancer cells. (C) Quantitative analysis of transfection efficiency in SKOV3 cells. (D) Western-blotting assays reflecting the expression level of MTH1 protein. (E) Representative photos of ovarian cancer cells after the in vivo treatment. Significant differences between groups were indicated as ** p < 0.01, and *** p < 0.001. Reproduced with permission from [40]. Copyright ACS Publications, 2017.