Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Oct 16;22(20):11180. doi: 10.3390/ijms222011180

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Therapy-induced effects on TAM accumulation and tumor vascularization. (a) Representative immunofluorescence staining showing no differences in TAM accumulation between groups (DAPI: blue, IBA1: green, scale bar = 100 µm). Additionally, no obvious changes in TAM morphology by therapy were observed as indicated in the magnified images. (b) The quantitative analysis of TAM accumulation showed comparable levels between all groups. (c) Representative immunofluorescence staining showing a tendency of diminished tumor vascularization (CD31: red, scale bar = 100 µm). (d–f) Quantitative comparison of vessel parameters showed reduced vessel area (calculated as a percentage in relation to the total analyzed tumor area; n = 4; Control vs. TMZ, p ≥ 0.9999; Control vs. TMZ + SB, p = 0.3951; TMZ vs. TMZ + SB, p = 0.8661) and average vessel size (calculated as total vessel area/vessel number; n = 4; Control vs. TMZ, p = 0.8802; Control vs. TMZ + SB, p = 0.2449; TMZ vs. TMZ + SB, p ≥ 0.9999) which was most markedly in the combination group. Vessel density was unchanged in all groups (n = 4; Control vs. TMZ, p = 0.1841; Control vs. TMZ + SB, p = 0.4623; TMZ vs. TMZ + SB, p ≥ 0.9999). Graphs depict individual values with additional mean ± standard deviation. One-way ANOVA with Bonferroni correction was performed in all analyses.

Therapy-induced effects on TAM accumulation and tumor vascularization. (a) Representative immunofluorescence staining showing no differences in TAM accumulation between groups (DAPI: blue, IBA1: green, scale bar = 100 µm). Additionally, no obvious changes in TAM morphology by therapy were observed as indicated in the magnified images. (b) The quantitative analysis of TAM accumulation showed comparable levels between all groups. (c) Representative immunofluorescence staining showing a tendency of diminished tumor vascularization (CD31: red, scale bar = 100 µm). (d–f) Quantitative comparison of vessel parameters showed reduced vessel area (calculated as a percentage in relation to the total analyzed tumor area; n = 4; Control vs. TMZ, p ≥ 0.9999; Control vs. TMZ + SB, p = 0.3951; TMZ vs. TMZ + SB, p = 0.8661) and average vessel size (calculated as total vessel area/vessel number; n = 4; Control vs. TMZ, p = 0.8802; Control vs. TMZ + SB, p = 0.2449; TMZ vs. TMZ + SB, p ≥ 0.9999) which was most markedly in the combination group. Vessel density was unchanged in all groups (n = 4; Control vs. TMZ, p = 0.1841; Control vs. TMZ + SB, p = 0.4623; TMZ vs. TMZ + SB, p ≥ 0.9999). Graphs depict individual values with additional mean ± standard deviation. One-way ANOVA with Bonferroni correction was performed in all analyses.