. 2021 Oct 14;10(10):2177. doi: 10.3390/plants10102177

Table 2.

Physiological plant traits of different barley genotypes 17 days after the onset of water stress in the rhizoboxes experiment.

Parameter		Treatment
Parameter	Genotype	WW	WS	p-Values	% Change
Chlorophyll-a (FW, µgmL⁻¹)	Barke	2.37 ± 0.3 ^a	1.07 ± 0.1 ^b	Genotype ***	−24
	Scarlett	2.14 ± 0.0 ^a	1.17 ± 0.0 ^b	Treatment ***	−19
	NIL 143	2.89 ± 0.2 ^a	2.69 ± 0.0 ^a	G × T *	−7
Fv/Fm	Barke	0.8 ± 0.0 ^a	0.81 ± 0.0 ^a	Genotype ^NS	0
	Scarlett	0.81 ± 0.0 ^a	0.81 ± 0.0 ^a	Treatment ^NS	0
	NIL 143	0.81 ± 0.0 ^a	0.81 ± 0.0 ^a	G × T ^NS	0
A (µmol m⁻² s⁻¹)	Barke	32.3 ± 0.3 ^a	12.0 ± 0.6 ^d	Genotype ***	−63
	Scarlett	27.5 ± 1.9 ^b	14.4 ± 0.3 ^d	Treatment ***	−48
	NIL 143	30.4 ± 0.9 ^ab	19.4 ± 0.4 ^c	G × T ***	−36
gsw (mol m⁻² s⁻¹)	Barke	0.602 ± 0.06 ^a	0.086 ± 0.01 ^c	Genotype *	−86
	Scarlett	0.533 ± 0.02 ^a	0.105 ± 0.02 ^c	Treatment ***	−80
	NIL 143	0.530 ± 0.02 ^a	0.183 ± 0.02 ^b	G × T ***	−65
E (mol m⁻² s⁻¹)	Barke	8.2 × 10⁻³ ± 1.7 × 10^{−4 a}	1.9 × 10⁻³ ± 2.3 × 10^{−4 c}	Genotype **	−77
	Scarlett	7.2 × 10⁻³ ± 1.5 × 10^{−4 a}	2.2 × 10⁻³ ± 4.9 × 10^{−4 c}	Treatment ***	−69
	NIL 143	7.8 × 10⁻³ ± 2.3 × 10^{−4 a}	3.7 × 10⁻³ ± 1.7 × 10^{−4 b}	G × T ***	−53
iWUE (µmol CO₂ mol⁻¹ H₂O)	Barke	52.6 ± 3.14 ^c	134.41 ± 3.14 ^a	Genotype **	−155
	Scarlett	54.5 ± 3.14 ^c	122.5 ± 3.14 ^a	Treatment ***	−125
	NIL 143	56.4 ± 3.44 ^c	104.2 ± 3.14 ^b	G × T ***	−85
Ci (μmol mol⁻¹)	Barke	277.9 ± 3.3 ^a	171.7 ± 7.1 ^c	Genotype ***	−178
	Scarlett	284.6 ± 5.1 ^a	183.4 ± 7.7 ^bc	Treatment *	−185
	NIL 143	279.0 ± 2.9 ^a	208.0 ± 6.9 ^b	G × T *	−179
Plant water potential, Ψ (Mpa)	Barke	−0.394 ± 0.2 ^ab	−1.23 ± 0.11 ^c	Genotype **	−75
	Scarlett	−0.293 ± 0.2 ^ab	−1.26 ± 0.11 ^c	Treatment ***	−121
	NIL 143	−0.170 ± 0.2 ^a	−0.17 ± 0.11 ^bc	G × T ^NS	−78
% RWC	Barke	98 ± 2.0 ^a	46 ± 1.8 ^c	Genotype *	−51
	Scarlett	88 ± 2.0 ^ab	41 ± 1.8 ^c	Treatment ***	−53
	NIL 143	95 ± 2.2 ^a	59 ± 1.7 ^b	G × T *	−41

Fv/Fm: maximum quantum efficiency of PSII, A: Net CO₂ assimilation, E: transpiration, gsw: stomatal conductance, iWUE (A/gsw): intrinsic water use efficiency, Ci: Intercellular CO₂, % RWC: percentage relative leaf water content, G × T: genotype × treatment interaction, FW: fresh weight. NS: no significance. Data are means ± standard error (n = 6) after the two-way ANOVA. Significant differences between the genotypes based on Tukey’s post hoc test (α = 0.05) are indicated with different letters. Asterisks: *, **, *** follows the standard probability values of 0.05, 0.01, and 0.001, respectively. The % change was calculated as the mean difference between drought and control conditions expressed as a percentage.