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. 2021 Oct 9;13(10):3537. doi: 10.3390/nu13103537

Figure 4.

Figure 4

Effects of 4,5-diCQA on LPS-induced activation of NF-κB and phosphorylation of MAPKs in RAW264.7 cells-stimulated with LPS. Cells were pretreated with 4,5-diCQA (1, 2, and 4 µM) for 1 h, followed by LPS (50 ng/mL) stimulation for 1 h. (A) Phosphorylation levels of IκB-α and NF-κB p65 translocation to nucleus were determined using Western blot analysis. (B) Protein levels of phosphorylation of MAPKs (ERK, JNK, and p38) were determined using Western blot analysis. α-Tubulin and Lamin B1 were used as cytosolic and nuclear internal controls, respectively. CON; control, IκB-α; nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha, p65; NF-kappa-B p65 subunit, ERK; extracellular signal-regulated kinase, JNK; c-Jun N-terminal kinase.