Table 1.

PCR primers used to amplify the partial 16S rRNA, groEL, and dsb genes of Ehrlichia spp.

Primer Name	Targeted Gene	Sequence (5′–3′)	Amplicon Size (bp)	Reference
EHR16SD * EHR16SR * fD1	16S rRNA	GGT ACC YAC AGA AGA AGT CC	345	[21]
		TAG CAC TCA TCG TTT ACA GC		[21]
		AGA GTT TGA TCC TGG CTC AG	~1431	[22,23]
rP2		ACG GCT ACC TTG TTA CGA CTT		[22,23]
HS1a	groEL	AIT GGG CTG GTA ITG AAA T	~1400	[24,26]
HS6a		CCI CCI GGI ACI AIA CCT TC		[24,26]
HS43		ATW GCW AAR GAA GCA TAG TC	1297	[25]
HSVR		CTC AAC AGC AGC TCT AGT AGC		[25]
Dsb-330	dsb	GAT GAT GTT TGA AGA TAT SAA ACA AAT	401	[27,28]
Dsb-720 ** Dsb-380		CTA TTT TAC TTC TTA AAG TTG ATA WAT C	349	[27,28]
		ATT TTT AGR GAT TTT CCA ATA CTT GG		[28]

* Primers used in the initial PCR screening, ** primer used on first and second round.