Table 2.
Compiled relative standard deviations of analyzed free fatty acids in biological matrices after manual and automated TMSH derivatization. Improvements in reproducibility are indicated with bolded %RSD values. NF = not found.
| Fatty Acid | Carbons:Double Bonds | HepG2 n = 6 | DU145 n = 6 | U937 n = 6 | FBS n = 6 | ||||
|---|---|---|---|---|---|---|---|---|---|
| Manual | Automatic | Manual | Automatic | Manual | Automatic | Manual | Automatic | ||
| Heptanoic acid | C7:0 | NF | NF | NF | NF | NF | NF | NF | NF |
| Octanoic acid | C8:0 | 12.29 | 6.74 | 10.80 | 5.16 | 7.88 | 5.33 | 24.24 | 18.95 |
| Nonanoic acid | C9:0 | NF | NF | NF | NF | NF | NF | NF | NF |
| Decanoic acid | C10:0 | 4.77 | 4.79 | 10.36 | 2.23 | 3.86 | 4.76 | 7.32 | 4.87 |
| Undecanoic acid | C11:0 | 4.13 | 3.68 | NF | NF | NF | NF | 6.52 | 5.87 |
| Dodecanoic acid | C12:0 | 3.33 | 2.55 | 5.29 | 2.94 | 3.15 | 4.48 | 7.18 | 5.63 |
| Tridecanoic acid | C13:0 | 5.44 | 7.24 | NF | NF | 14.27 | 11.59 | 21.36 | 23.31 |
| 9(Z)-Tetradecenoic acid | C14:1 | NF | NF | NF | NF | NF | NF | NF | NF |
| Tetradecanoic acid | C14:0 | 5.23 | 5.43 | NF | NF | 3.02 | 4.06 | 8.70 | 5.09 |
| 9(Z)-Pentedecenoic acid | C15:1 | 3.81 | 8.69 | 51.44 | 5.88 | 6.97 | 3.00 | 5.50 | 12.39 |
| Pentadecanoic acid | C15:0 | 6.17 | 7.41 | 41.89 | 21.60 | 3.13 | 4.66 | 11.34 | 7.35 |
| 9(Z)-Hexadecenoic acid | C16:1 | 3.37 | 3.19 | 30.09 | 17.32 | 2.26 | 1.82 | 19.98 | 7.27 |
| Hexadecanoic acid | C16:0 | 3.25 | 5.00 | 7.93 | 11.17 | 2.19 | 3.28 | 6.83 | 5.04 |
| 10(Z)-Heptadecenoic acid | C17:1 | 4.79 | 14.33 | NF | NF | 4.51 | 3.04 | 9.08 | 9.14 |
| Heptadecanoic acid | C17:0 | 6.49 | 3.79 | 19.65 | 7.05 | 1.00 | 1.03 | 4.69 | 2.69 |
| (6Z, 9Z, 12Z)-Octadecatrienoic acid | C18:3 | NF | NF | NF | NF | 14.21 | 19.39 | NF | NF |
| (9Z, 12Z)-octadecadienoic acid | C18:2 | NF | NF | NF | NF | 5.42 | 2.74 | 20.74 | 5.43 |
| 9(Z)-octadecenoic acid | C18:1 | 1.26 | 6.18 | 8.15 | 5.88 | 1.25 | 1.28 | 15.88 | 4.60 |
| Octadecanoic acid | C18:0 | 9.03 | 2.78 | 11.31 | 7.59 | 1.79 | 2.04 | 5.28 | 4.33 |
| (5Z, 8Z, 11Z, 14Z)-Icosatetraenoic acid | C20:4 | 14.34 | 15.46 | 71.96 | 28.56 | 9.21 | 3.77 | NF | NF |
| (5Z, 8Z, 11Z)-Eicosatrienoic acid | C20:3 | 11.10 | 15.08 | 40.73 | 20.70 | 10.79 | 5.57 | NF | NF |
| (11Z, 14Z)-Eicosadienoic acid | C20:2 | 7.70 | 12.23 | 18.63 | 12.29 | NF | NF | NF | NF |
| 13(Z)-Eicosenoic acid | C20:1 | 6.19 | 12.72 | 11.22 | 4.70 | 7.81 | 4.96 | NF | NF |
| Eicosanoic acid | C20:0 | 27.20 | 5.50 | 10.44 | 4.78 | 5.07 | 3.46 | 44.30 | 29.24 |
| Heinecosanoic acid | C21:0 | NF | NF | NF | NF | NF | NF | NF | NF |
| (4Z, 7Z, 10Z, 13Z, 16Z, 19Z)-Docosahexaenoic acid | C22:6 | 22.28 | 14.46 | NF | NF | 6.48 | 5.94 | NF | NF |
| (13Z)-Docosenoic acid | C22:1 | NF | NF | 11.85 | 9.28 | NF | NF | NF | NF |
| Docosanoic acid | C22:0 | 8.70 | 20.28 | 11.27 | 3.11 | 5.10 | 6.38 | 28.19 | 17.57 |
| Tricosanoic acid | C23:0 | NF | NF | 47.77 | 14.83 | 8.81 | 6.36 | NF | NF |
| (15Z)-Tetracosenoic acid | C24:1 | 8.30 | 14.30 | 12.74 | 6.92 | 8.66 | 3.37 | 42.72 | 6.93 |
| Tetracosanoic acid | C24:0 | 6.59 | 33.25 | 4.77 | 4.48 | 8.20 | 2.44 | 44.78 | 7.33 |
| Hexacosanoic acid | C26:0 | 10.07 | 7.00 | 8.50 | 7.60 | 6.25 | 3.08 | 11.95 | 7.41 |