Figure 5.

Individual effects of polyhydroxysteroid glycosides from P. lincki and sulfated derivative of laminaran from A. angusta on the invasion of 3D HCT 116 spheroids. The 3D HCT 116 spheroids were treated with (A) PBS (control) (B) PL1, (C) PL2, (D) L1 (12.5, 25, 50 μM) or (E) AaLs (200, 400, 800 μg/mL) after 72 h of invasion into the ECM gel. Photos of spheroids were made using a Motic microscope (40× magnification, 500 μm scale). (F) Quantitative measurement of the invasion area of spheroids treated with individual compounds was carried out using the ImageJ program and determined as the difference between total area invaded by cells leaving the spheroid and the area of the spheroid. All analyses were performed in three independent experiments. Results are expressed as mean ± standard deviation (SD). The Student’s t test was used to evaluate data with the following levels of significance *** p < 0.001.