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Modification of phi11 genome (A) showing holin and amidase genes (B) and creation of SASPject PT1.2 by deletion of holin gene and promoter driving holin and amidase genes by insertion of SASP-C gene under the control of native S. aureus promoter, fbaA and, selectable marker, cadmium resistance gene (C). Phi11 genome visualized using CGView [21].
