Figure 11.
Assessment of resistance development in serially passaged EMRSA strain O2ST4285 by time-kill curve assay of cells from passage day 52 (3 and 6 × 104 pfu/mL passaged cultures) and passage day 46 (1.5 × 104 pfu/mL passaged cultures) exposed to SASPject PT1.2. Passaging was carried out in triplicate, and time-kill curve assays were carried out in singlicate on each of the triplicate cultures. Cells that were passaged in singlicate in the absence of PT1.2 for 52 days were exposed to PT1.2 as a control (Control). The mean number of viable cells for each passaging condition following time-kill curve assay with PT1.2 is plotted. Error bars for the passaged conditions represent 1 standard deviation.