Figure 12.

Assessment of resistance development in serially passaged MRSA USA300 by exposing passaged cells to PT1.2 at passage days 7, 14, and 21, and incubating with PT1.2 for 4 h before assessing viable cell count. Triplicate cultures were set up for each of the phage concentrations, and each culture was assessed via the kill experiment in singlicate. Results shown are the mean viable cell counts for the three cultures passaged with each PT1.2 concentration, following kill experiment analysis. Error bars represent 1 standard deviation. The control was MRSA USA300 simultaneously passaged for the same lengths of time in the absence of PT1.2, prior to assessment of viable cell count after exposure to PT1.2 for 4 h.