Figure 6.

Confirmation of HA expression in S2 cells. Day three success of HA expression in supernatant of S2 cell culture confirmed by green fluorescent protein expression control (at a magnification of ×20) displaying about >90% transfection efficiency (A); ELISA, showing similar expression patterns for cHA-C, cHA-E and CVV-M controlled by an in-house H5 HA expressing-S2 cells, and supernatant from an un-transfected cell control (UTC) (B) or Western blotting showing blots of cHA-C, cHA-E and CVV-M at expected band sizes of approximately 75 kD (C).