Table 1.
Effect of cRG-I on microbial composition at family level in the lumen (log cells/mL as estimated via multiplying total cell counts (cells/mL) with 16S rRNA gene profiling (%)) and mucus (%) of the simulated proximal (PC) and distal colon (DC) of the quad-M-SHIME. Values presented are the averages of the differences at the end of the treatment period (Day 35) versus those at the end of the control period (Day 14) across the four human adult donors tested (n = 4). As a result, positive or negative values indicate families that are increased or decreased, respectively, upon cRG-I treatment. Statistical differences are indicated in bold.
| Phylum | Family | Lumen | Mucus | ||
|---|---|---|---|---|---|
| PC | DC | PC | DC | ||
| Actinobacteria | Atopobiaceae | 0.43 | 0.30 | 0.11% | 0.02% |
| Bifidobacteriaceae | 0.30 | 0.02 | −6.00% | 3.98% | |
| Coriobacteriaceae | 0.23 | −0.02 | 2.16% | −0.41% | |
| Eggerthellaceae | 0.00 | −0.17 | 0.00% | −0.02% | |
| Bacteroidetes | Bacteroidaceae | 0.61 | 0.33 | 3.99% | −2.81% |
| Barnesiellaceae | 0.00 | 0.27 | 0.00% | 0.05% | |
| Marinifilaceae | 0.00 | 0.01 | 0.00% | 0.01% | |
| Muribaculaceae | 0.00 | −0.01 | 0.00% | 0.02% | |
| Prevotellaceae | 0.52 | 0.69 | 7.55% | 1.85% | |
| Rikenellaceae | −0.02 | 0.07 | −0.07% | −0.30% | |
| Tannerellaceae | 0.38 | −0.10 | −0.12% | −0.03% | |
| Firmicutes | Acidaminococcaceae | 0.47 | 0.39 | 0.46% | 0.16% |
| Christensenellaceae | 0.00 | 0.44 | 0.01% | 0.21% | |
| Clostridiaceae | 0.00 | 0.00 | −0.01% | −1.00% | |
| Erysipelotrichaceae | 0.16 | 0.68 | 0.22% | 0.18% | |
| Family XIII | 0.00 | 0.01 | 0.00% | −0.03% | |
| Lachnospiraceae | 0.24 | 0.19 | −3.83% | 0.96% | |
| Lactobacillaceae | 0.00 | 0.03 | −0.15% | 0.02% | |
| Ruminococcaceae | 0.43 | −0.21 | 0.08% | −1.15% | |
| Staphylococcaceae | 0.03 | 0.19 | 0.01% | 0.07% | |
| Veillonellaceae | −0.08 | −0.13 | −7.19% | 1.41% | |
| Proteobacteria | Burkholderiaceae | 0.18 | 0.12 | −0.36% | −0.36% |
| Desulfovibrionaceae | −0.28 | −0.06 | −0.60% | −0.75% | |
| Enterobacteriaceae | 0.06 | 0.25 | 0.32% | −0.27% | |
| Pseudomonadaceae | 0.00 | 0.00 | −0.01% | 0.00% | |
| uncultured | 0.00 | 0.64 | 0.00% | 0.20% | |
| Xanthomonadaceae | 0.39 | 0.42 | 0.04% | 0.00% | |
| Synergistetes | Synergistaceae | 0.54 | −0.09 | 3.36% | −0.22% |
| Verrucomicrobia | Akkermansiaceae | 0.00 | −0.09 | 0.00% | −0.01% |