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. 2021 Oct 1;13(10):1975. doi: 10.3390/v13101975

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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ML phylogenetic tree of coronaviruses. Sequences were aligned using the G-INS1 and default parameters implemented in Mafft using online tool: https://mafft.cbrc.jp/alignment/server [50]. ML phylogenetic trees were inferred using PhyML (version 3.0) implemented in Seaview (Lyon, France), employing the GTR+G4 substitution model, a heuristic SPR branch-swapping algorithm, and SH-like branch supports [51]; obtained trees were edited online for graphical display using iTOL on server: https://itol.embl.de/ [52]. CoV species investigated in the study are indicated in red, with branches of tested strains shown as red boxes.